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Image Search Results
Journal: The FEBS journal
Article Title: Nuclear patterns of phosphatidylinositol 4,5- and 3,4-bisphosphate revealed by super-resolution microscopy differ between the consecutive stages of RNA polymerase II transcription.
doi: 10.1111/febs.17136
Figure Lengend Snippet: Fig. 1. Correlation of the consecutive stages of RNAPII transcription and nPIPs. Transcription initiation and elongation marker P-S5 and P-S2, respectively, and nuclear PI(4,5)P2 or PI(3,4)P2 indirectly immunolabeled with AF647 (magenta) and AF555 (green), respectively, with zoom-in to the boxed ROIs in the nucleoplasm (Np) or nuclear speckles (Sp) imaged by dSTORM (A). Normalized control (ctrl) and random (rand.) NND distributions between P-S5 or P-S2 and nPI(4,5)P2 or nPI(3,4)P2 in the Np and Sp ROIs (B). Color-coded pixel maps of the NND between P-S5 or P-S2 and nPI(4,5)P2 or nPI(3,4)P2 (C) with zoom-in to the boxed ROIs. (D) The Mode NNDs and the Fraction of the NNDs in the mode NND in the Np and at Sp ROIs. Scale bars = 5 lm; zoom-in = 1 lm. Measurements P-S5 to PI(4,5)P2 in the Np are from 28 and in Sp from 29 ROIs from n = 12 nuclei from N = 4 independent experiments; P-S2 to PI(4,5)P2 Np and Sp both 32 ROIs n = 14, N = 3; P-S5 to PI(3,4)P2 Np 27 and Sp 30 ROIs n = 11, N = 3; P-S2 to PI(3,4)P2 Np 43 and Sp 36 ROIs n = 8, N = 3. The error bars in the normalized NND distributions in (B) represent the SEM. The data in (D) are plotted as Tukey whisker plots and were evaluated statistically using one-way ANOVA followed by Bonferoni post-test expressing the statistical significance: **P < 0.01; ***P < 0.005.
Article Snippet: The secondary antibodies and concentrations used were: goat anti-mouse IgM (l-chain),
Techniques: Marker, Immunolabeling, Control, Whisker Assay, Expressing